NMR conformational analysis of biosynthetic precursor-type lipid A: monomolecular state and supramolecular assembly.

نویسندگان

  • Masato Oikawa
  • Tetsuya Shintaku
  • Naohiro Fukuda
  • Harald Sekljic
  • Yoshiyuki Fukase
  • Hiroaki Yoshizaki
  • Koichi Fukase
  • Shoichi Kusumoto
چکیده

The detailed conformational analysis of a single molecule of the tetraacyl biosynthetic precursor-type lipid A and its characteristic supramolecular assembly in aqueous SDS-micelles are described. Regular molecular arrangements were observed by detailed analysis of the NMR spectra of synthetically pure specimens, including regiospecifically 13C-labeled ones. NMR analysis of a biologically inactive precursor-type analogue with four shorter acyl chains demonstrated its conformational flexibility, indicating the importance of hydrophobic interactions for maintaining the conformation of such molecules.

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عنوان ژورنال:
  • Organic & biomolecular chemistry

دوره 2 24  شماره 

صفحات  -

تاریخ انتشار 2004